General Cell Based Assays
Eurofins Discoverx PathHunter Human S1P3 (EDG3) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human S1P3 (EDG3) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure EDG3 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress CHRM4 Total Internalization Assay, 10pl Kit. (93-0902E3CP0L)
The PathHunter CHRM4 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM4 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CHRM4 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CHRM4 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR75 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0363E2ACP1L)
The PathHunter GPR75 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR75 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR75 and EA-tagged β-Arrestin-2. Activation of GPR75 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CCR9 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0296E2CP0M)
The PathHunter CCR9 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL25) based activation of CCR9 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR9 and EA-tagged β-Arrestin-2. Activation of CCR9 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam NADH disodium salt trihydrate, Coenzyme, 1G
MW 763.4 g/mol, Purity >95%. Sodium salt of NADH, a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). Also participates in cell signaling events, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress AVPR1A U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0587E3CP0M)
The PathHunter AVPR1A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. vasopressin) based activation of AVPR1A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AVPR1A and EA-tagged β-Arrestin-2. Activation of AVPR1A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Mouse Ghrelin (GHSR) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse Ghrelin (GHSR) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GHSR1a (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress mCMKLR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0537E2MCP0M)
The PathHunter mCMKLR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. chemerin) based activation of mCMKLR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCMKLR1 and EA-tagged β-Arrestin-2. Activation of mCMKLR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OPRD1 Activated GPCR Internalization Assay, 10pl Kit. (93-0673E3CP0L)
The PathHunter OPRD1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of OPRD1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged OPRD1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting OPRD1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human IL-37 ASY
The Meso Scale Discovery R-PLEX Human IL-37 Assay is a multiplex immunoassay that measures IL-37 levels in human serum plasma and cell culture supernatants It uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample consumption This assay allows for the simultaneous measurement of IL-37 and other biomarkers It requires 50 L or less of sample and can be read on the MESO QuickPlex SQ 120 instrument The assay comes with a plate coated with capture antibodies detection antibodies calibrators and controls
Eurofins Discoverx cAMP Hunter eXpress OPRK1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0088E2CP2L)
The cAMP Hunter OPRK1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dynorphin A) based activation of OPRK1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type OPRK1, a naturally Gi/Go coupled GPCR. Upon activation of OPRK1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Genscript Corporation CHO-K1/AGTRL1/G15 Stable Cell
Recombinant CHO-K1 cells stably overexpress human apelin receptor (AGTRL1) on the surface and contain high levels of G protein G i to couple with the receptor in downstream signaling pathways
Eurofins Discoverx PathHunter eXpress β-Catenin Nuclear Translocation Assay, 10pl Kit. (93-0743E3CP22L)
The PathHunter eXpress β-Catenin Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the Wnt-FzGSK3β pathway. This kit assesses ligand (e.g. wnt 3a) based pathway activation via detection of resulting β-Catenin nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged β-Catenin and nuclear localized EA. Upon pathway activation, induced nuclear translocation of β-Catenin leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.